restriction endonuclease是什麼意思，restriction endonuclease的意思翻譯、用法、同義詞、例句

限制酶，限制性内切核酸酶

The recombinant plasmid PET- 11D-ospC was identified with restriction endonuclease analysis and sequencing.

采用酶切分析及序列測定等方法鑒定重組質粒的正确性。

In this study, a detailed restriction map was constructed using multiple groups of restriction endonuclease analysis.

本研究利用多種組合的酶切分析，繪制了較為詳細的酶切圖譜。

Results PCR, restriction endonuclease method and direct sequence analysis demonstrated that plasmids of YMDD, YVDD and YIDD were constructed successfully.

結果經PCR方法、酶切鑒定及直接測序鑒定出YMDD、YVDD和YIDD質粒構建成功，且該方法具有較好的敏感性和特異性。

Methods PCR and DNA sequencing were performed to study the AR gene mutation; Mbo I restriction endonuclease was used to detect existence of the mutation in normal controls;

并對發現突變的基因進行分析。方法 應用PCR擴增、DNA序列測定等技術分析所有AR基因外顯子及其鄰近DNA序列片段；

Restriction enzyme:Protein (more specifically, an endonuclease) produced by bacteria that cleaves DNA at specific sites along its length.

限制性内切：由細菌産生的一種蛋白質，能在特定的地方切斷去氧核糖核酸分子。 收藏。

Finally, we could evaluate plasmids cDNA extracted with mono-restriction endonuclease enzyme and the agar gel electrophoresis.

最後用限制性内切酶單酶切及瓊脂凝膠電泳進行鑒定。

Methods:DNA sequencing and restriction endonuclease reaction was used.

方法：采用DNA測序及限制性酶切反應方法。

Finally, the cloned B antigen gene was identified by restriction endonuclease analysis, DIG probe hybridization and DNA sequencing.

通過内切酶分析、*********（DIG）—探針原位雜交、DNA序列測定，确認克隆的B抗原基因正确。

Janpanese encephalitis virus; recombinant plasmid; DNA sequencing; restriction endonuclease analysis.

流行性乙型腦炎病毒；重組質粒；DNA測序；限制性内切酶分析。

CP4-EPSPS gene was amplified from 13 samples, but all of them were proved to be false positives in restriction endonuclease digestion analysis of PCR products.

有13個樣品在CP4-EPSPS基因檢測中雖擴增出類似陽性對照的條帶，但對PCR産物的進一步酶切鑒定表明，所有擴增結果為假陽性。

Result Restriction endonuclease digestion and sequencing showed that the modification of the sense mutation of OSCP gene can be successful.

結果酶切及測序顯示對OSCP基因有義突變部分的糾正獲得成功。

Methods DNAs were extracted from the white blood cells of people in Hainan by salt-out method. Polymerase chain reaction and restriction endonuclease was used to determine the 4533G/A polymorphism.

方法用鹽提取法提取人群中白細胞的DNA，以聚合酶鍊反應、限制性内切核酸酶檢測-4533G/A多态性。

Secondly, the preamplified DNA fragments were digested by a restriction endonuclease to form sticky ends, which were then ligated to a designed DNA adapter by ligase.

然後用限制性内切酶将其消化成短片段，在連接酶的作用下與設計的DNA適配器相連；

The overlap extension mediated by restriction endonuclease to obtain the full length gene was established.

建立一種用於克隆全長基因的、限制性内切酶介導的重疊延伸法 。

Then, we analyzed the specificity of PCR primers through digesting PCR products with restriction endonuclease.

然後對擴增産物進行限制性酶切反應；

After a restriction endonuclease cleavage, two expected smaller fragments were observed.

經限制性内切酶反應，獲得兩個預期大小的片段。

Result The recombinant was correctly constructed and restriction endonuclease analysis and PCR amplification.

結果 經酶切鑒定及基因測序證實重組三突變型穿梭質粒構建成功。

|restriction enzyme;限制酶，限制性内切核酸酶