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[生化] 辣根*********物酶
Objective:To clone and express the horseradish peroxidase isozyme C3(HRPC3) gene.
目的：克隆与表达辣根*********物酶同功酶C3（HRPC3）基因。
Based on the inhibition of cyanide to the horseradish peroxidase(HRP), the HRP electrode has been used in the determination of cyanide in water.
基于*********对辣根*********物酶（HRP）的抑制作用，将辣根*********物酶电极用于水中的微量*********的测定。
Unilateral injection of horseradish peroxidase into the muscle resulted in bilateral labelling of cell in the ventromedial region of the caudal trigeminal nucleus.
将辣根*********物酶注入单侧肌肉内，在三叉神经运动核尾侧的腹内侧部引起了双侧的标记细胞。
The Electrochemical behavior of the product, 2,3 diaminophenazine (DAP), of the enzymatic catalytic reaction by horseradish peroxidase was stu***d by various electrochemical methods.
用多种电化学分析方法研究了辣根*********物酶催化反应产物2 ，3-*********吩嗪（DAP）的电化学行为。
This Cell Signaling Technology (CST) antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups.
CST公司的该抗体通过其氨基结合了辣根*********物酶（HRP）的碳水化合物基团。
Afferent connections of the oaudoputamen in the rat were stu***d by axonal retrograde transport method using horseradish peroxidase (HRP).
应用辣根*********物酶（HRP）的逆行传递技术研究了大鼠尾壳复合体的传入性联系。
After the horseradish peroxidase (HRP) was treated with different strengths of electric field, the HRP and activity index were assayed by ultraviolet-visible spectra.
用不同电场强度处理辣根*********物酶，测试电场处理前后的紫外-可见光谱和酶的活力指数。
Horseradish peroxidase was used to label the regenerative axons of peroneal nerves 4 or 9 weeks after epineurial or Perineurial suture or nerve grafting in sciatic nerves in rats.
大鼠坐骨神经外膜缝合、束膜缝合或神经移植后4周或9周，应用辣根*********物酶逆行标记腓总神经中再生轴突的胞体。
The distribution of lectin binding in gastrointestinal tract of human, rat and mouse was investigated using horseradish peroxidase labeled lectins-WGA, SBA, and PNA staining method.
用三种辣根*********物酶标记的凝集素—WGA、SBA和PNA观察人及大小鼠的消化管各段中凝集素结合部位的分布。
Transepithelial electrical resistance(TER)was detected and the permeability was measured with fluorescein sodium(FS)and horseradish peroxidase(HRP).
行跨膜电阻测定并用荧光素钠和辣根*********物酶（HRP）进行通透性检测。
The reaction between the TMB substrate and a peroxidase, typically horseradish peroxidase (HRP), produces a measureable color change that correlates with analyte level.
TMB与*********物酶，典型的是辣根*********物酶（HRP）之间的反应可以产生可测量的与分析物相关颜色变化。
Insulin was labeled with horseradish peroxidase by sodium periodate oxidation method.
用*********钠氧化法将辣根*********物酶标记于胰岛素。
Thyroxine (T_4) was coupled with horseradish Peroxidase(HRP) by a two-step linking method of glutaraldehyde.
采用*********二步法使辣根*********物酶（HRP）与半抗原T_4相偶联。
Lignin and phenol was copolymerized with horseradish peroxidase as catalyst in reversed micelles, and the copolymer was analyzed by infra red and differential scanning calorimetry.
在反相微乳液系统中，用辣根*********物酶进行了酶促木质素与酚共聚反应，并进行了红外光谱分析及差示扫描量热分析。
The monoclonal antibody against IL-2 was labelled with horseradish peroxidase.
用辣根*********物酶标记IL-2单克隆抗体。
A double substrates enzyme reaction, which horseradish peroxidase (HRP) catalytic oxide of o -phenylenediamine (OPD) by H2O2, were stu***d by thermokinetic method.
利用微量热方法研究了辣根*********物酶（HRP）催化H_2O_2氧化************（OPD）的反应。
The distribution of lectin receptor in liver and pancreas of rats and mice was investigated using WGA, SBA, PNA labeled with horseradish peroxidase.
本文用辣根*********物酶标记的麦芽凝集素、大豆凝集素及花生凝集素来研究大、小鼠肝、胰中凝集素受体的分布。
A voltammetric enzyme - linked immunoassay based on a new system of o- tilidine (OT) - H2O2 - horseradish peroxidase (HRP) has firstly been developed.
本文首次提出邻联*********（OT）-H_2O_2-辣根*********物酶（HRP）伏安酶联免疫分析新体系。
A pair of redox peaks attributed to the direct redox reaction of horseradish peroxidase in the phosphate buffer solution was observed.
结果显示：在磷酸缓冲溶液中，辣根*********物酶在钛酸纳米管修饰的玻碳电极上呈现出一对良好的氧化还原峰。
Distribution of LH cells in the anterior pituitary of the female rats were stu***d using the horseradish peroxidase immunohistochemical method.
应用辣根*********物酶标记抗体技术，对正常和阉割成年雌性大白鼠垂体LH细胞的分布进行了研究。
Horseradish peroxidase was immobilized by bovine serum albumin and glutaraldehyde on the surface of the glassy carbon electrode, which was modified with hydroquinone.
用牛血清白蛋白和*********把辣根*********物酶固定在************修饰的电极上，制成*********氢传感器。
AGE-BSA of different concentration was applied to BREC in different time. And Horseradish peroxidase (HRP) engulfed by BREC was surveyed.
检测不同浓度AGE-BSA在不同时间对BREC内吞辣根*********物酶（HRP）情况。
Anterior pagoda (AP) neurons of leeches were marked by intracellular injection of horseradish peroxidase (HRP).
用胞内注射辣根*********物酶（HRP）的方法标记中华宽体金线蛭AP神经元。
Taking horseradish peroxidase (HRP) as an enzyme model, this presented platform has been successfully exploited to develop a novel electrochemical enzyme sensor for the detection of H2O2.
以固定辣根*********物酶（HRP）为例，发展了一种新的电化学酶传感器用于H2O2的测定。
Anti-biotin (D5A7) Rabbit mAb is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups.
抗生物素（D5A7）兔源抗体（HRP标记）可以与辣根*********物酶通过气氨基基团与碳水化合物基团结合。
The development of antibody—producing immunocytes was examined in the popliteal lymph nodes of the guinea pig during their primary response to horseradish peroxidase (HRP).
本文以辣根*********物酶（HRP）与福氏完全佐剂混合成的乳液对雄性豚鼠作一次免疫，追踪腘淋巴结中抗体形成细胞的分化途径和变化。
辣根过氧化物酶（Horseradish Peroxidase, HRP） 是一种广泛存在于辣根（Armoracia rusticana）等植物中的氧化还原酶，属于过氧化物酶超家族。其核心功能是催化过氧化物（如过氧化氢，H₂O₂）对多种有机和无机底物的氧化反应，同时伴随发色团或荧光团的显色变化。以下是其详细解释：
酶学特性
HRP是一种糖蛋白，分子量约44 kDa，含有一个高自旋铁（Ⅲ）原卟啉IX（血红素）辅基作为活性中心。其催化机制依赖于血红素铁离子的价态变化（Fe³⁺ ↔ Fe⁴⁺），通过中间体化合物I和II实现电子转移。典型反应式为：
$$ ce{ROOR' + 2e^- + 2H+ -> ROH + R'OH} $$
其中ROOR'代表过氧化物底物（如H₂O₂）。
底物特异性
HRP可催化多种供氢体（如邻苯二胺、四甲基联苯胺）的氧化，产生有色或荧光产物。例如：
免疫检测技术
HRP是酶联免疫吸附试验（ELISA）和免疫印迹（Western Blot）的关键标记酶。其偶联于抗体后，可通过底物显色定量检测抗原，灵敏度达皮摩尔级别。例如，COVID-19抗体检测试剂盒常采用HRP-抗体复合物。
分子生物学探针
在原位杂交和荧光原位杂交（FISH）中，HRP标记的核酸探针可催化酪胺信号放大（TSA），实现低丰度靶标的可视化检测。
生物传感器与诊断
HRP用于构建电化学生物传感器，通过检测H₂O₂浓度间接定量葡萄糖、胆固醇等代谢物。其高催化效率（k_cat≈10⁷ s⁻¹）使其成为理想的生物标志物放大器。
HRP在辣根中作为防御蛋白，通过氧化酚类物质生成醌类毒素抵御病原体侵染。其天然丰度高（占可溶性蛋白的~1%），且具有耐热性（最适温度40-50℃）和pH稳定性（pH 5-9），适合工业化纯化与应用。
酶学机制与结构
应用技术
植物生理学背景
horseradish peroxidase（HRP） 是一种重要的酶类，以下为详细解释：
定义与来源
HRP是从辣根（Amoracia rusticana）根部提取的过氧化物酶，属于铁原卟啉酶类，由酶蛋白和铁卟啉辅基结合而成。其英文缩写为HRP（Horseradish Peroxidase）。
结构与特性
应用领域
主要用于免疫组化中标记抗原与抗体，也用于生物化学实验的催化反应（如ELISA检测）。此外，可通过化学修饰（如炔基化）扩展其功能。
其他补充
HRP的辅基为棕色的铁卟啉，而酶蛋白部分呈无色，整体为糖蛋白结构。
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