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restriction endonuclease是什么意思,restriction endonuclease的意思翻译、用法、同义词、例句

输入单词

常用词典

  • 限制酶,限制性内切核酸酶

  • 例句

  • The recombinant plasmid PET- 11D-ospC was identified with restriction endonuclease analysis and sequencing.

    采用酶切分析及序列测定等方法鉴定重组质粒的正确性。

  • In this study, a detailed restriction map was constructed using multiple groups of restriction endonuclease analysis.

    本研究利用多种组合的酶切分析,绘制了较为详细的酶切图谱。

  • Results PCR, restriction endonuclease method and direct sequence analysis demonstrated that plasmids of YMDD, YVDD and YIDD were constructed successfully.

    结果经PCR方法、酶切鉴定及直接测序鉴定出YMDD、YVDD和YIDD质粒构建成功,且该方法具有较好的敏感性和特异性。

  • Methods PCR and DNA sequencing were performed to study the AR gene mutation; Mbo I restriction endonuclease was used to detect existence of the mutation in normal controls;

    并对发现突变的基因进行分析。方法 应用PCR扩增、DNA序列测定等技术分析所有AR基因外显子及其邻近DNA序列片段;

  • Restriction enzyme:Protein (more specifically, an endonuclease) produced by bacteria that cleaves DNA at specific sites along its length.

    限制性内切:由细菌产生的一种蛋白质,能在特定的地方切断去氧核糖核酸分子。 收藏。

  • Finally, we could evaluate plasmids cDNA extracted with mono-restriction endonuclease enzyme and the agar gel electrophoresis.

    最后用限制性内切酶单酶切及琼脂凝胶电泳进行鉴定。

  • Methods:DNA sequencing and restriction endonuclease reaction was used.

    方法:采用DNA测序及限制性酶切反应方法。

  • Finally, the cloned B antigen gene was identified by restriction endonuclease analysis, DIG probe hybridization and DNA sequencing.

    通过内切酶分析、地高辛(DIG)—探针原位杂交、DNA序列测定,确认克隆的B抗原基因正确。

  • Janpanese encephalitis virus; recombinant plasmid; DNA sequencing; restriction endonuclease analysis.

    流行性乙型脑炎病毒;重组质粒;DNA测序;限制性内切酶分析。

  • CP4-EPSPS gene was amplified from 13 samples, but all of them were proved to be false positives in restriction endonuclease digestion analysis of PCR products.

    有13个样品在CP4-EPSPS基因检测中虽扩增出类似阳性对照的条带,但对PCR产物的进一步酶切鉴定表明,所有扩增结果为假阳性。

  • Result Restriction endonuclease digestion and sequencing showed that the modification of the sense mutation of OSCP gene can be successful.

    结果酶切及测序显示对OSCP基因有义突变部分的纠正获得成功。

  • Methods DNAs were extracted from the white blood cells of people in Hainan by salt-out method. Polymerase chain reaction and restriction endonuclease was used to determine the 4533G/A polymorphism.

    方法用盐提取法提取人群中白细胞的DNA,以聚合酶链反应、限制性内切核酸酶检测-4533G/A多态性。

  • Secondly, the preamplified DNA fragments were digested by a restriction endonuclease to form sticky ends, which were then ligated to a designed DNA adapter by ligase.

    然后用限制性内切酶将其消化成短片段,在连接酶的作用下与设计的DNA适配器相连;

  • The overlap extension mediated by restriction endonuclease to obtain the full length gene was established.

    建立一种用于克隆全长基因的、限制性内切酶介导的重叠延伸法 。

  • Then, we analyzed the specificity of PCR primers through digesting PCR products with restriction endonuclease.

    然后对扩增产物进行限制性酶切反应;

  • After a restriction endonuclease cleavage, two expected smaller fragments were observed.

    经限制性内切酶反应,获得两个预期大小的片段。

  • Result The recombinant was correctly constructed and restriction endonuclease analysis and PCR amplification.

    结果 经酶切鉴定及基因测序证实重组三突变型穿梭质粒构建成功。

  • 同义词

  • |restriction enzyme;限制酶,限制性内切核酸酶

  • 网络扩展资料

    restriction

    解释: 限制,限制性规定,限制性条件

    例句:

    • The new law imposes restrictions on smoking in public places.(新法律对公共场所的吸烟进行限制。)
    • There are some restrictions on the use of this equipment.(对于使用这种设备有一些限制。)

    用法: 作为名词使用,表示对某物的限制。

    近义词: limitation, constraint, regulation, control

    反义词: allowance, freedom, liberty, permission

    endonuclease

    解释: 内切酶,一种能够切割DNA或RNA链的酶。

    例句:

    • Endonucleases are important tools in genetic engineering.(内切酶是基因工程中重要的工具。)
    • The endonuclease cleaves the DNA molecule at a specific site.(内切酶在特定位点切割DNA分子。)

    用法: 作为名词使用,表示一种酶类。

    近义词: nuclease, exonuclease, RNase

    反义词: N/A

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